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Volume 7, Issue 2 (2026)
3 2026, 7(2): 101-106 |
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Received: 2026/03/10 | Accepted: 2026/06/9 | Published: 2026/06/21
Received: 2026/03/10 | Accepted: 2026/06/9 | Published: 2026/06/21
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Naser H, Al-Semaiil F, Mohammed M, Abbas B. Isolation and Identification of Proteus mirabilis from Clinical Samples Using the VITEK-2 Automated System. 3 2026; 7 (2) :101-106
URL: http://jccs.daneshafarand.org/article-8-87254-en.html
URL: http://jccs.daneshafarand.org/article-8-87254-en.html
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1- Department of Pathological Analyses, Faculty of Science, Al-Kunooze University, Basra, Iraq
2- Department of Science, College of Education Open (Al-Dujail Study Branch), Al-Dujail, Iraq
3- Department of Pathological Analysis, Faculty of Health and Medical Techniques, Al-Kunooze University, Basra, Iraq, Department of Pathological Analysis, Faculty of Health and Medical Techniques, Al-Kunooze University, Street/Al-Zaytoon, Basra, Iraq. Postal Code: 610001 (basilabbas63@gmail.com)
2- Department of Science, College of Education Open (Al-Dujail Study Branch), Al-Dujail, Iraq
3- Department of Pathological Analysis, Faculty of Health and Medical Techniques, Al-Kunooze University, Basra, Iraq, Department of Pathological Analysis, Faculty of Health and Medical Techniques, Al-Kunooze University, Street/Al-Zaytoon, Basra, Iraq. Postal Code: 610001 (basilabbas63@gmail.com)
Abstract (79 Views)
Aims: This study aimed to systematically characterize the phenotypic profiles of clinical Proteus mirabilis isolates identified using the VITEK 2 GN card and to determine the consistency and intraspecies variability of the biochemical reactions underlying their identification.
Materials & Methods: This descriptive observational study was conducted in 2025. Twenty-three clinical Gram-negative isolates obtained from over 150 urine samples were identified using the VITEK 2 GN card. Identification probability, bionumbers, analysis time, and the binary results of 47 biochemical reactions were obtained from the instrument reports. The isolates identified as Proteus mirabilis were further evaluated based on their individual biochemical profiles to determine the consistency and variability of the detected reactions.
Findings: VITEK 2 identified 18/23 isolates (78.3%) with probabilities ≥90%, whereas 5/23 (21.7%) were reported as “Unidentified Organism.” Proteus mirabilis was the most frequent identification (9/23; 39.1%); eight isolates showed 99% probability, and one showed 96%, with a mean analysis time of 3.84 hours. All Proteus mirabilis isolates were positive for H₂S, D-glucose, D-trehalose, phosphatase, coumarate, and O/129 resistance. ODC was positive in 8/9 (88.9%), whereas LDC was negative in all isolates. Urease and γ-glutamyl transferase were positive in 7/9 (77.8%) and 8/9 (88.9%) of the isolates, respectively, whereas tyrosine arylamidase and citrate utilization showed greater variability. Nine distinct bionumbers indicated limited biochemical variation among the isolates.
Conclusion: The VITEK 2 GN card provides high-confidence identification of Proteus mirabilis and demonstrates a consistent biochemical profile across the isolates.
Materials & Methods: This descriptive observational study was conducted in 2025. Twenty-three clinical Gram-negative isolates obtained from over 150 urine samples were identified using the VITEK 2 GN card. Identification probability, bionumbers, analysis time, and the binary results of 47 biochemical reactions were obtained from the instrument reports. The isolates identified as Proteus mirabilis were further evaluated based on their individual biochemical profiles to determine the consistency and variability of the detected reactions.
Findings: VITEK 2 identified 18/23 isolates (78.3%) with probabilities ≥90%, whereas 5/23 (21.7%) were reported as “Unidentified Organism.” Proteus mirabilis was the most frequent identification (9/23; 39.1%); eight isolates showed 99% probability, and one showed 96%, with a mean analysis time of 3.84 hours. All Proteus mirabilis isolates were positive for H₂S, D-glucose, D-trehalose, phosphatase, coumarate, and O/129 resistance. ODC was positive in 8/9 (88.9%), whereas LDC was negative in all isolates. Urease and γ-glutamyl transferase were positive in 7/9 (77.8%) and 8/9 (88.9%) of the isolates, respectively, whereas tyrosine arylamidase and citrate utilization showed greater variability. Nine distinct bionumbers indicated limited biochemical variation among the isolates.
Conclusion: The VITEK 2 GN card provides high-confidence identification of Proteus mirabilis and demonstrates a consistent biochemical profile across the isolates.
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