Received: 2025/05/23 | Accepted: 2025/07/1 | Published: 2025/07/5
URL: http://jccs.daneshafarand.org/article-8-87179-en.html
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, A. Abdollahi2 , P. Afarinesh Khaki3 , M. Norouzi Shadehi3 , M.T. Beighmohammadi4 , S. Nateghi5 , Z. Panahi6 , A. Nozheh *1
2- Department of Pathology, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran
3- Central laboratory of Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran
4- Department of Intensive Care Medicine, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran
5- Department of Cardiology, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran
6- Department of Obstetrics and Gynecology, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran
Aims: Rising antibiotic resistance among prevalent pathogens poses a critical challenge, especially in intensive care unit patients. This study aimed to evaluate antibiotic susceptibility and resistance patterns in body fluid and urine samples from intensive care unit patients at a tertiary care center in Tehran, Iran.
Instrument & Methods: This cross-sectional study analyzed cultures from body fluids and urine samples collected from intensive care unit patients at Imam Khomeini Hospital between 2019 and 2022. Cultures were processed according to standard protocols and interpreted based on growth inhibition zones to classify bacterial isolates as sensitive, intermediate, or resistant. Data analysis was performed using STATA 17.
Findings: A total of 4,232 body fluid and 4,062 urine samples were examined; 2,400 body fluid and 2,824 urine cultures were negative. The most common bacteria in urine were Klebsiella pneumoniae (16.7%), Escherichia coli (15.43%), and Pseudomonas aeruginosa (6.08%), while body fluids predominantly grew Klebsiella pneumoniae (37.19%), Acinetobacter baumannii (25.52%), and Pseudomonas aeruginosa (8%). Klebsiella pneumoniae showed the highest sensitivity to Gentamicin (12.37% in body fluids, 17.93% in urine) and imipenem (9.72% in body fluids, 14.67% in urine). Resistance was notably high against ampicillin-sulbactam (over 90%), ciprofloxacin (85-89%), and cotrimoxazole or nitrofurantoin (84-88%), depending on the sample type.
Conclusion: Klebsiella pneumoniae, Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus have a high resistance against many antimicrobial agents.